abstract：:Endothelial barrier dysfunction leads to edema and vascular leak, causing high morbidity and mortality. Previously, Abl kinase inhibition has been shown to protect against vascular leak. Using the distinct inhibitory profiles of clinically available Abl kinase inhibitors, we aimed to provide a mechanistic basis for no...

journal_title：Journal of cell science

authors： Botros L,Pronk MCA,Juschten J,Liddle J,Morsing SKH,van Buul JD,Bates RH,Tuinman PR,van Bezu JSM,Huveneers S,Bogaard HJ,van Hinsbergh VWM,Hordijk PL,Aman J

更新日期：2020-05-14 00:00:00

abstract：:Actin polymerization is a crucial process during sperm capacitation. We have recently described the participation of FAK during actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK mediates these processes is unknown. Our previous data have shown that MAPK1 (hereafter referred to as ERK2...

journal_title：Journal of cell science

authors： Salgado-Lucio ML,Ramírez-Ramírez D,Jorge-Cruz CY,Roa-Espitia AL,Hernández-González EO

更新日期：2020-04-28 00:00:00

abstract：:Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response, viral infections, DNA damage responses and apoptosis. To date, 28 HECT E3 ubiquitin ligases have been identifi...

journal_title：Journal of cell science

authors： Wang Y,Argiles-Castillo D,Kane EI,Zhou A,Spratt DE

更新日期：2020-04-07 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:Signalling by target-derived neurotrophins is essential for the correct development of the nervous system and its maintenance throughout life. Several aspects concerning the lifecycle of neurotrophins and their receptors have been characterised over the years, including the formation, endocytosis and trafficking of si...

journal_title：Journal of cell science

authors： Budzinska MI,Villarroel-Campos D,Golding M,Weston A,Collinson L,Snijders AP,Schiavo G

更新日期：2020-03-30 00:00:00

abstract：:Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed....

journal_title：Journal of cell science

authors： Neubert E,Meyer D,Kruss S,Erpenbeck L

更新日期：2020-03-10 00:00:00

abstract：:The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the cont...

journal_title：Journal of cell science

authors： Girón-Pérez DA,Vadillo E,Schnoor M,Santos-Argumedo L

更新日期：2020-03-02 00:00:00

abstract：:The phagocytic ability of macrophages empowers them to enforce innate immunity. RAW264.7, THP-1 and peripheral blood mononuclear cell-derived macrophages display considerable variability with regards to their phagocytic ability. We identify the underlying causes that attenuate the phagocytic abilities of a macrophage....

journal_title：Journal of cell science

authors： Agarwal M,Biswas P,Bhattacharya A,Sinha DK

更新日期：2020-03-02 00:00:00

abstract：:Fluorescence illumination can cause phototoxicity that negatively affects living samples. This study demonstrates that much of the phototoxicity and photobleaching experienced with live-cell fluorescence imaging occurs as a result of 'illumination overhead' (IO). This occurs when a sample is illuminated but fluorescen...

journal_title：Journal of cell science

authors： Kiepas A,Voorand E,Mubaid F,Siegel PM,Brown CM

更新日期：2020-02-21 00:00:00

abstract：:Motile eukaryotic flagella beat through coordinated activity of dynein motor proteins; however, the mechanisms of dynein coordination and regulation are incompletely understood. The inner dynein arm (IDA) f complex (also known as the I1 complex), and the tether and tether head (T/TH) complex are thought to be key regu...

journal_title：Journal of cell science

authors： Beneke T,Banecki K,Fochler S,Gluenz E

更新日期：2020-01-23 00:00:00

abstract：:High-throughput neurotransmission at ribbon synapses of cochlear inner hair cells (IHCs) requires tight coupling of neurotransmitter release and balanced recycling of synaptic vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) fo...

journal_title：Journal of cell science

authors： Kroll J,Özçete ÖD,Jung S,Maritzen T,Milosevic I,Wichmann C,Moser T

更新日期：2020-01-22 00:00:00

abstract：:Mechanical cues are essential for the regulation of cell and tissue physiology. Hence, it has become an utmost necessity for cell biologists to account for those mechanical parameters when investigating biological processes and they need devices to manipulate cells accordingly. Here, we report a simple mechanical cell...

journal_title：Journal of cell science

authors： Boulter E,Tissot FS,Dilly J,Pisano S,Féral CC

更新日期：2020-01-06 00:00:00

abstract：:Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...

journal_title：Journal of cell science

authors： Xiong J,He J,Xie WP,Hinojosa E,Ambati CSR,Putluri N,Kim HE,Zhu MX,Du G

更新日期：2019-12-13 00:00:00

abstract：:Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...

journal_title：Journal of cell science

authors： Singh RK,Lund FW,Haka AS,Maxfield FR

更新日期：2019-12-02 00:00:00

abstract：:The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...

journal_title：Journal of cell science

authors： Altman T,Geller D,Kleeblatt E,Gradus-Perry T,Perlson E

更新日期：2019-12-02 00:00:00

abstract：:Mammalian, or mechanistic, target of rapamycin complex 2 (mTORC2) regulates a variety of vital cellular processes, and its aberrant functioning is often associated with various diseases. Rictor is a peculiar and distinguishing mTORC2 component playing a pivotal role in controlling its assembly and activity. Among exta...

journal_title：Journal of cell science

authors： Pergolizzi B,Panuzzo C,Ali MS,Lo Iacono M,Levra Levron C,Ponzone L,Prelli M,Cilloni D,Calautti E,Bozzaro S,Bracco E

更新日期：2019-11-14 00:00:00

abstract：:The mechanistic target of rapamycin (mTOR) signaling pathway coordinates environmental and intracellular cues to control eukaryotic cell growth. As a pivot point between anabolic and catabolic processes, mTOR complex 1 (mTORC1) signaling has established roles in regulating metabolism, translation and autophagy. Hypera...

journal_title：Journal of cell science

authors： Condon KJ,Sabatini DM

更新日期：2019-11-13 00:00:00

abstract：:Cells depend on a highly ordered organisation of their content and must develop strategies to maintain the anisotropic distribution of organelles during periods of nutrient shortage. One of these strategies is to solidify the cytoplasm, which was observed in bacteria and yeast cells with acutely interrupted energy pro...

journal_title：Journal of cell science

authors： Heimlicher MB,Bächler M,Liu M,Ibeneche-Nnewihe C,Florin EL,Hoenger A,Brunner D

更新日期：2019-11-08 00:00:00

abstract：:During animal development, cells need to sense and adapt to mechanical forces from their environment. Ultimately, these forces are transduced through the actomyosin cortex. How the cortex simultaneously responds to and creates forces during cytokinesis is not well understood. Here we show that, under mechanical stress...

journal_title：Journal of cell science

authors： Singh D,Odedra D,Dutta P,Pohl C

更新日期：2019-10-09 00:00:00

abstract：:Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. ...

journal_title：Journal of cell science

authors： Hasegawa J,Jebri I,Yamamoto H,Tsujita K,Tokuda E,Shibata H,Maki M,Itoh T

更新日期：2019-10-03 00:00:00

abstract：:Intermediate filament protein keratin 8 (K8) binds to heat shock protein 70 (Hsp70) and p38 MAPK, and is phosphorylated at Ser74 by p38α (MAPK14, hereafter p38). However, a p38 binding site on K8 and the molecular mechanism of K8-p38 interaction related to Hsp70 are unknown. Here, we identify a p38 docking site on K8 ...

journal_title：Journal of cell science

authors： Lee SY,Kim S,Lim Y,Yoon HN,Ku NO

更新日期：2019-09-26 00:00:00

abstract：:Recent studies show that mitochondria and actin filaments work together in two contexts: (1) increased cytoplasmic calcium induces cytoplasmic actin polymerization that stimulates mitochondrial fission and (2) mitochondrial depolarization causes actin assembly around mitochondria, with roles in mitophagy. It is unclea...

journal_title：Journal of cell science

authors： Fung TS,Ji WK,Higgs HN,Chakrabarti R

更新日期：2019-09-23 00:00:00

abstract：:What forces drive chromosome segregation remains one of the most challenging questions in cell division. Even though the duration of anaphase is short, it is of utmost importance for genome fidelity that no mistakes are made. Seminal studies in model organisms have revealed different mechanisms operating during chromo...

journal_title：Journal of cell science

authors： Vukušić K,Buđa R,Tolić IM

更新日期：2019-09-16 00:00:00

abstract：:How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of...

journal_title：Journal of cell science

authors： Sengupta S,Rothenberg KE,Li H,Hoffman BD,Bursac N

更新日期：2019-09-02 00:00:00

abstract：:Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor ...

journal_title：Journal of cell science

authors： Prasad S,Ponimaskin E,Zeug A

更新日期：2019-08-23 00:00:00

abstract：:Motile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BBs) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associa...

journal_title：Journal of cell science

authors： Junker AD,Soh AWJ,O'Toole ET,Meehl JB,Guha M,Winey M,Honts JE,Gaertig J,Pearson CG

更新日期：2019-08-07 00:00:00

abstract：:Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...

journal_title：Journal of cell science

authors： R Ferreira R,Fukui H,Chow R,Vilfan A,Vermot J

更新日期：2019-07-30 00:00:00

abstract：:Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to ...

journal_title：Journal of cell science

authors： Wilton KM,Overlee BL,Billadeau DD

更新日期：2019-07-18 00:00:00

abstract：:Interactions between different cell types can induce distinct contact inhibition of locomotion (CIL) responses that are hypothesised to control population-wide behaviours during embryogenesis. However, our understanding of the signals that lead to cell-type specific repulsion and the precise capacity of heterotypic CI...

journal_title：Journal of cell science

authors： Brayford S,Kenny FN,Hiratsuka T,Serna-Morales E,Yolland L,Luchici A,Stramer BM

更新日期：2019-05-31 00:00:00

abstract：:During mitosis, the cell sequentially constructs two microtubule-based spindles to ensure faithful segregation of chromosomes. A bipolar spindle first pulls apart the sister chromatids, then a central spindle further separates them away. Although the assembly of the first spindle is well described, the assembly of the...

journal_title：Journal of cell science

authors： Courthéoux T,Reboutier D,Vazeille T,Cremet JY,Benaud C,Vernos I,Prigent C

更新日期：2019-05-16 00:00:00

abstract：:Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely respons...

journal_title：Journal of cell science

authors： Tsygankova OM,Keen JH

更新日期：2019-05-15 00:00:00

abstract：:Previously, we documented the role of the programmed death-1 (PD-1, also known as PDCD1) pathway in macrophage apoptosis and the downregulation of this signaling during infection by the intra-macrophage parasite Leishmania donovani However, we also found that, during the late phase of infection, PD-1 expression was si...

journal_title：Journal of cell science

authors： Roy S,Saha S,Gupta P,Ukil A,Das PK

更新日期：2019-05-02 00:00:00

abstract：:It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms by which MTs and F-actin are dually coordinated remain a fundamentally unresolved question. Here, we report that the well-characterized ...

journal_title：Journal of cell science

authors： Slater PG,Cammarata GM,Samuelson AG,Magee A,Hu Y,Lowery LA

更新日期：2019-04-30 00:00:00

abstract：:Sustained neurotransmission requires the tight coupling of synaptic vesicle (SV) exocytosis and endocytosis. The mechanisms underlying this coupling are poorly understood. We tested the hypothesis that a cGMP-dependent protein kinase (PKG), encoded by the foraging (for) gene in Drosophila melanogaster, is critical for...

journal_title：Journal of cell science

authors： Dason JS,Allen AM,Vasquez OE,Sokolowski MB

更新日期：2019-04-05 00:00:00

abstract：:The GGGGCC (G4C2) repeat expansion mutation in the C9ORF72 gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Transcription of the repeat and formation of nuclear RNA foci, which sequester specific RNA-binding proteins, is one of the possible pathological me...

journal_title：Journal of cell science

authors： Bajc Česnik A,Darovic S,Prpar Mihevc S,Štalekar M,Malnar M,Motaln H,Lee YB,Mazej J,Pohleven J,Grosch M,Modic M,Fonovič M,Turk B,Drukker M,Shaw CE,Rogelj B

更新日期：2019-03-07 00:00:00

abstract：:Actin cytoskeleton self-organization in two cell types, fibroblasts and epitheliocytes, was studied in cells confined to isotropic adhesive islands. In fibroblasts plated onto islands of optimal size, an initially circular actin pattern evolves into a radial pattern of actin bundles that undergo asymmetric chiral swir...

journal_title：Journal of cell science

authors： Jalal S,Shi S,Acharya V,Huang RY,Viasnoff V,Bershadsky AD,Tee YH

更新日期：2019-03-07 00:00:00

abstract：:The metabolism of PI(3,5)P2 is regulated by the PIKfyve, VAC14 and FIG4 complex, mutations in which are associated with hypopigmentation in mice. These pigmentation defects indicate a key, but as yet unexplored, physiological relevance of this complex in the biogenesis of melanosomes. Here, we show that PIKfyve activi...

journal_title：Journal of cell science

authors： Bissig C,Croisé P,Heiligenstein X,Hurbain I,Lenk GM,Kaufman E,Sannerud R,Annaert W,Meisler MH,Weisman LS,Raposo G,van Niel G

更新日期：2019-02-28 00:00:00

abstract：:In mammals, fertilization initiates Ca2+ oscillations in metaphase II oocytes, which are required for the activation of embryo development. Germinal vesicle (GV) oocytes also display Ca2+ oscillations, although these unfold spontaneously in the absence of any known agonist(s) and their function remains unclear. We fou...

journal_title：Journal of cell science

authors： Wakai T,Fissore RA

更新日期：2019-02-12 00:00:00

abstract：:The essential function of the T cell receptor (TCR) is to translate the engagement of peptides on the major histocompatibility complex (pMHC) into appropriate intracellular signals through the associated cluster of differentiation 3 (CD3) complex. The spatial organization of the TCR-CD3 complex in the membrane is thou...

journal_title：Journal of cell science

authors： Goyette J,Nieves DJ,Ma Y,Gaus K

更新日期：2019-02-11 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00